d13C

Delta-C-13; δ13C

Measure for the enrichment of the stable carbon isotope 13C in the food web

Carbon has two stable isotopes, 13C and 12C. They differ in their atomic masses and can therefore be detected separately by means of mass spectrometry (isotope ratio MS). The lighter isotope 12C is more frequent constituting 98.9% of the total carbon whereas 13C contributes only with 1.1%.

In nature, the ratio of 13C to 12C depends mainly on the photosynthesis efficiency of plants.
The lighter isotope 12C reacts faster and its enzymatic transformation is more rapid. This leads to an enrichment of the heavier isotope 13C in organisms. This enrichment progresses in the food web.

The differences between ratios are normally small. They are considered in relation to one another using the so-called δ-notation (δ13C), the values being expressed as per mille (‰). An increase in δ13C indicates an increase of 13C accumulation in the sample.
The δ13C values of organisms are influenced by their immediate environment and their food and can therefore provide information on the origin of samples and the nutrition of organisms.
In aquatic ecosystems, the δ13C value can be used to distinguish between the two major energy sources – attached algae and detritus on the one hand and phytoplankton on the other. This helps to assign organisms either to the littoral or the pelagic food web.

Specimen

Sampling area

Sampling period

1988 - 2024